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How does ROSALIND calibrate multi-lot and PlexSet datasets?

For scenarios where a user has more than one lot of the same panel or for PlexSet data, the user is able to define reference or calibration samples in ROSALIND during experiment setup. These samples are used to quantify and adjust for variability in probe efficiency across batches or lanes. Calibration factors are calculated on a per lot basis and are multiplied across all probes in that lot.