Abundance of various cell populations is calculated on ROSALIND using the Cell Type Profiling Module. The method quantifies cell populations using marker genes which are expressed stably and specifically in given cell types. ROSALIND performs a filtering of Cell Type Profiling results to include results that have scores with a p-Value less than or equal to 0.05. Cell Type Profiling Algorithm details can be found on page 84 of the nCounter® Advanced Analysis 2.0 User Manual.
Here's a short tutorial on our Term Exploration/Cell Type Profiler. We include topics like how to interpret your heatmap, how to filter the list of cell types, and how to change the data display of any specific cell type.